Depends on where you are staying.
When you register, clarify your room rate per day. Ask for the breakdown (taxes, etc.). If a Resort Fee is part of it, ask what that includes. Typically they quote stuff that is free to hotel guests anyway, such as "use of the pool [well, yeah], use of the fitness room [usually free anyway], daily newspaper [wooo], free local calls from your room [who doesn't have a cell phone to do that], etc.
If free Internet is included in the Resort Fee, that is of some value and it may make the resort fee a break even situation in that case.
In times past, some had success getting the resort fee removed at registration by saying "I won't be using any of those things so please remove the fee." But that may be a thing of the past. Doesn't hurt to ask, though.
Up front comped rooms usually have no resort fee attached.
I doubt you can get the resort fee removed at the end of your stay. It will be assumed that you used all the "benefits", or if you didn't that was your loss. But you can always see a Host and ask.
Just look at it as part of the cost of your Vegas vacation and don't let it give you heartburn.
